Vitamin D, 25-Hydroxy

Vitamin D, 25-Hydroxy

$ 55.99

Doctor’s order included No insurance needed No additional lab fees

Pricing is all-inclusive. No surprise lab or collection fees. Orders and results are sent electronically.

Vitamin D, 1,25-Dihydroxy LC/MS/MS Blood Test

Active Vitamin D Calcitriol Test for Calcium, Kidney and Parathyroid Evaluation

The Vitamin D, 1,25-Dihydroxy LC/MS/MS Blood Test measures the active form of vitamin D in the blood, also known as calcitriol.

This test is best used when a healthcare provider specifically requests active vitamin D testing for calcium metabolism, kidney-related vitamin D processing, parathyroid concerns, or other focused medical evaluation.

Important: This is not the standard vitamin D deficiency test. If you want to check routine vitamin D deficiency or supplement status, you likely need the Vitamin D, 25-Hydroxy test instead.

✔ Measures active vitamin D, also called calcitriol
✔ LC/MS/MS testing method
✔ Useful when specifically requested by your provider
✔ Doctor's order included
✔ No insurance required

What This Test Measures

This blood test measures 1,25-dihydroxyvitamin D, the active hormone form of vitamin D. Vitamin D is first converted in the liver to 25-hydroxyvitamin D and then converted mainly in the kidneys to 1,25-dihydroxyvitamin D.

  • Test name: Vitamin D, 1,25-Dihydroxy LC/MS/MS
  • Also known as: Calcitriol, 1,25-Dihydroxy Vitamin D, 1,25 di-OH Vitamin D
  • Sample type: Blood
  • Purpose: Active vitamin D evaluation

Vitamin D 1,25 vs Vitamin D 25-Hydroxy

  • Vitamin D, 25-Hydroxy - Best for routine vitamin D deficiency, sufficiency, and supplement monitoring.
  • Vitamin D, 1,25-Dihydroxy - Best for active vitamin D evaluation related to calcium, kidney, parathyroid, hypercalcemia, or special provider-directed testing.

A person can have a normal Vitamin D, 1,25-Dihydroxy result even when overall vitamin D stores are low. If you are unsure which vitamin D test you need, confirm with your healthcare provider before ordering.

Why Order This Test?

  • Provider specifically requested it - This test is most appropriate when your provider asks for active vitamin D or calcitriol testing.
  • Calcium metabolism evaluation - May be used when calcium levels are abnormal.
  • Kidney-related evaluation - May be used when kidney function affects vitamin D activation.
  • Parathyroid-related evaluation - May help support review of parathyroid-related concerns.
  • Specialized vitamin D workup - May be used in focused bone, mineral, or metabolic evaluations.

Who May Consider This Test?

  • Individuals whose provider requested Vitamin D, 1,25-Dihydroxy testing
  • Patients being evaluated for certain calcium abnormalities
  • Patients being evaluated for parathyroid-related concerns
  • Individuals with kidney-related vitamin D metabolism concerns
  • Patients being monitored for specific bone or mineral metabolism concerns

Important Limitations

  • This test does not diagnose vitamin D deficiency by itself.
  • This test does not replace Vitamin D, 25-Hydroxy for routine deficiency screening.
  • A normal result does not rule out low vitamin D stores.
  • This test does not diagnose kidney disease, parathyroid disease, sarcoidosis, lymphoma, or any medical condition by itself.
  • Results should be reviewed with your healthcare provider along with calcium, kidney function, parathyroid hormone, symptoms, and other lab findings.

Before Ordering

  • Confirm that your provider requested Vitamin D, 1,25-Dihydroxy, not Vitamin D, 25-Hydroxy.
  • If your goal is routine vitamin D deficiency screening, consider ordering Vitamin D, 25-Hydroxy instead.
  • Do not stop supplements or medications unless your healthcare provider tells you to.
  • If you are unsure which vitamin D test is right for you, ask your healthcare provider before ordering.

How It Works

  1. Order online.
  2. Receive your lab order. A doctor's order is included.
  3. Visit a participating lab location.
  4. Complete a simple blood draw.
  5. Receive your results. Result timing may vary by lab, location, and processing requirements.

Why Choose This Test?

✅ Measures active vitamin D, also called calcitriol
✅ Useful for provider-directed calcium, kidney, or parathyroid evaluation
✅ Doctor's order included
✅ No insurance needed
✅ Convenient online ordering
✅ Results can be reviewed with your healthcare provider

Frequently Asked Questions

What does this test check?

This test checks Vitamin D, 1,25-Dihydroxy, the active form of vitamin D also called calcitriol.

Is this the regular vitamin D test?

No. The regular vitamin D test used for most deficiency screening is Vitamin D, 25-Hydroxy.

Should I order this for vitamin D deficiency?

Usually no. Vitamin D, 25-Hydroxy is generally the preferred test for vitamin D deficiency screening.

Why would my provider order this test?

Your provider may order this test for calcium, kidney, parathyroid, bone, or mineral metabolism evaluation.

Do I need to fast?

Fasting is usually not required unless your healthcare provider gives you different instructions.

Is a doctor's order included?

Yes. A doctor's order is included with this lab test.

Order Your Vitamin D, 1,25-Dihydroxy LC/MS/MS Test

Use this test when your provider specifically requests active vitamin D testing for calcitriol, calcium metabolism, kidney-related concerns, parathyroid-related concerns, or other focused medical review.

Order your Vitamin D, 1,25-Dihydroxy LC/MS/MS Blood Test today.

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Vitamin D, 25-Hydroxy
$ 55.99